| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145021 |
| Chromosome: | chromosome 10 |
| Location: | 44285 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g417750 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTTGGGGTGCTGCAAAAGGACCGAGAC |
| Internal bar code: | CTTCCGGGGGTTTATACGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 99.0752 |
| LEAP-Seq n confirming: | 9320 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCTCAAACGCTTAGGGC |
| Suggested primer 2: | ATACTGGTGGTCAAGTCGCC |