| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145037 |
| Chromosome: | chromosome 7 |
| Location: | 894551 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318800 | HSP22A | (1 of 2) K13993 - HSP20 family protein (HSP20); heat shock protein 22A | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCGGGAGGGAGGGAGCACAGGCGGGGA |
| Internal bar code: | GACTGGTAGGTCCCGGATTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1118 |
| LEAP-Seq percent confirming: | 99.6637 |
| LEAP-Seq n confirming: | 8593 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAAGTCGGGCTAGCTTTA |
| Suggested primer 2: | ACGAGGGCAGCATATGTAGG |