Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.145056 |
Chromosome: | chromosome 12 |
Location: | 9171160 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g542200 | 3'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCTAACTTGGGCTTACTTGTATCACTT |
Internal bar code: | GGCATAACTTTCCACAGCGATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1139 |
LEAP-Seq percent confirming: | 99.7856 |
LEAP-Seq n confirming: | 6517 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGACAGCCCTTACACGGATA |
Suggested primer 2: | TGGGATGTAGAGGTTGAGGC |