| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145063 |
| Chromosome: | chromosome 3 |
| Location: | 7426117 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCTTCAAGGCGCGGCTGAGCTCCAGCG |
| Internal bar code: | TGGTTCGGAGCGTGCGGTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 99.7361 |
| LEAP-Seq n confirming: | 756 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCTTGTCAGGTAGCCTC |
| Suggested primer 2: | CGATGGTTGTAACCCTGCTT |