Insertion junction: LMJ.RY0402.145070_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.145070
Chromosome:chromosome 11
Location:1036509
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467670 GT90F44,GT90-44 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 44 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAACCCTCCCGACGTCAAAGTCCGTGTG
Internal bar code:GGTGACCGCCACGTTGCGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:976
LEAP-Seq percent confirming:92.7021
LEAP-Seq n confirming:6707
LEAP-Seq n nonconfirming:528
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGCAACAAGTAAGAACAA
Suggested primer 2:AGTTATGGGTGGTTTGCAGC