Insertion junction: LMJ.RY0402.145073_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.145073
Chromosome:chromosome 6
Location:7364132
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g299150 PHC9,PHC26 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 26 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACACTGTTTCTGGCGACCAGCTTGTCGA
Internal bar code:GGCGGCTTCTTCGGAGTTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:439
LEAP-Seq percent confirming:32.0658
LEAP-Seq n confirming:2243
LEAP-Seq n nonconfirming:4752
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACTGGCACGGTAGGTGT
Suggested primer 2:ATTGCAAACACAAAGAGGGG