| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145077 |
| Chromosome: | chromosome 5 |
| Location: | 2460822 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235500 | KIN7A,KIN7-1 | Kinesin motor protein; (1 of 1) PTHR24115//PTHR24115:SF70 - FAMILY NOT NAMED // KINESIN-RELATED PROTEIN 11 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCAGCATCCCTGCCGTCCAGGTGTTGC |
| Internal bar code: | CCGAACAGTCAATTTTGTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1057 |
| LEAP-Seq percent confirming: | 99.5316 |
| LEAP-Seq n confirming: | 5737 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGAGCTTCCAAAACAAC |
| Suggested primer 2: | TACGGTACTGAAGTTCCCCG |