Insertion junction: LMJ.RY0402.145081_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.145081
Chromosome:chromosome 9
Location:2121348
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393650 (1 of 1) PF00069//PF01590 - Protein kinase domain (Pkinase) // GAF domain (GAF) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAGGCCTCCTTAAATCGTCCACCTGCT
Internal bar code:CGGGTTCGACTAGTGCGAGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1004
LEAP-Seq percent confirming:99.384
LEAP-Seq n confirming:7906
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGATTCGCCTACACACT
Suggested primer 2:TTGCAGACTTGATTGCTTGG