Insertion junction: LMJ.RY0402.145081_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.145081
Chromosome:chromosome 9
Location:2121348
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393650 (1 of 1) PF00069//PF01590 - Protein kinase domain (Pkinase) // GAF domain (GAF) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTAAATTATGTATGGGTACGGCGGGGAG
Internal bar code:CTTAACCGATCACGGTATAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:868
LEAP-Seq percent confirming:97.2103
LEAP-Seq n confirming:906
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGATTCGCCTACACACT
Suggested primer 2:TTGCAGACTTGATTGCTTGG