| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145103 |
| Chromosome: | chromosome 3 |
| Location: | 4840922 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178950 | HEL17 | (1 of 1) PTHR13710//PTHR13710:SF70 - DNA HELICASE RECQ FAMILY MEMBER // BLOOM SYNDROME PROTEIN; DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGCGGCGGGTAGCTCGCTATTCGTTGA |
| Internal bar code: | TGAGGAACAAGTGGGTGGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 86.4865 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCAAGTCCATAACCGCGT |
| Suggested primer 2: | GACGCGTTAAGAAGGAGTCG |