| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145126 |
| Chromosome: | chromosome 9 |
| Location: | 3271110 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387986 | 3'UTR|outside_mRNA | ||
| Cre09.g388023 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACACAAGCACGCAAGGCTAATGCGTTTA |
| Internal bar code: | CGGTATTAAGCGCAATTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 467 |
| LEAP-Seq percent confirming: | 99.2291 |
| LEAP-Seq n confirming: | 13386 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAAAACAACAACTTGGG |
| Suggested primer 2: | CTCACACTGGCAGAACTGGA |