| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145141 |
| Chromosome: | chromosome 10 |
| Location: | 4216980 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450100 | (1 of 1) K04957 - hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGGAAACAAAGGGTCACTTGCGTAAT |
| Internal bar code: | CGCAGACATCGAGGGCGTCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 842 |
| LEAP-Seq percent confirming: | 99.5334 |
| LEAP-Seq n confirming: | 1280 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGTGAGAGCACACACTC |
| Suggested primer 2: | CCTCGGGTACATCTAGCAGC |