Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.145142 |
Chromosome: | chromosome_3 |
Location: | 2076512 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g156700 | FAP185 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGCGCGGTTCTTGCGGCTGTTGCTGCCCGT |
Internal bar code: | AATTGGGAGGAAATATGTCAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2 |
LEAP-Seq percent confirming: | 99.4709 |
LEAP-Seq n confirming: | 564 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATACAACCACTCTCGGGCAC |
Suggested primer 2: | CGCTATCACCGTTAGCAACA |