| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145146 |
| Chromosome: | chromosome 12 |
| Location: | 99763 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484050 | RPL36 | Ribosomal protein L36, component of cytosolic 80S ribosome and 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCTGACGTTGCCCGACCCCTTCACCC |
| Internal bar code: | TTCGTTGTAATATCAGTCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1213 |
| LEAP-Seq percent confirming: | 97.6953 |
| LEAP-Seq n confirming: | 9580 |
| LEAP-Seq n nonconfirming: | 226 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCGCAAGATGAAGAAG |
| Suggested primer 2: | ATCCTGACTCCATTGCAACC |