Insertion junction: LMJ.RY0402.145149_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.145149
Chromosome:chromosome 7
Location:4631847
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g344260 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGTGGTCCAGAACACATGTGAAAGTAAT
Internal bar code:CATGCGGAGGACTCAGGGAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1119
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTCATCTGCCCCGTGTAAGT
Suggested primer 2:CAGAACTCGGCTGTGTTTGA