| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145150 |
| Chromosome: | chromosome 6 |
| Location: | 172070 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250300 | DHC16,DHC1b,FLA24 | (1 of 1) K10414 - dynein heavy chain 2, cytosolic (DYNC2H, DNCH2); Cytoplasmic Dynein 1b Heavy Chain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACAGCTGTTCAGGTCCATTGCCATGAC |
| Internal bar code: | GTGGGCGGCCTCCGACTCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 99.692 |
| LEAP-Seq n confirming: | 5827 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGCCTCTCAGTTCCAGC |
| Suggested primer 2: | GTACCTGGCCAAGGACAAAA |