| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145172 |
| Chromosome: | chromosome 10 |
| Location: | 678032 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422300 | PRX6 | (1 of 2) K03564 - peroxiredoxin Q/BCP (BCP, PRXQ, DOT5); Peroxiredoxin Q | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGTACTACGGCAGGTAGGGTACAACGA |
| Internal bar code: | CGATTTGTCTCGCGTTCATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 869 |
| LEAP-Seq percent confirming: | 99.5151 |
| LEAP-Seq n confirming: | 7593 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGATCTGGGTGGTTGGTCT |
| Suggested primer 2: | GAGCAACACCTCAAGGCTTC |