| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145181 |
| Chromosome: | chromosome 6 |
| Location: | 2929805 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272701 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATCCTTAGCTACTGCGCCGCTTCTTAG |
| Internal bar code: | GACCTGTCGTTGCTGCCGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 99.7111 |
| LEAP-Seq n confirming: | 2071 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTTAGTGGGGCGACAGTG |
| Suggested primer 2: | CTACACCGTGGGGCTACACT |