| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145181 |
| Chromosome: | chromosome 12 |
| Location: | 2422550 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507051 | (1 of 2) K01193 - beta-fructofuranosidase (E3.2.1.26, sacA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGACCCTCACCGCCCCCGCACCAGGTCG |
| Internal bar code: | GCCGCGCCAGGATAGCGCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 162 |
| LEAP-Seq percent confirming: | 99.8596 |
| LEAP-Seq n confirming: | 711 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGATTTCAACACACCTGG |
| Suggested primer 2: | AGCGATGAAGTGCATGAGTG |