| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145184 |
| Chromosome: | chromosome 6 |
| Location: | 5754298 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287176 | CPLD67 | dihydrolipoyllysine-residue acetyltransferase; (1 of 5) PF01713 - Smr domain (Smr) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGCGGCCATACAGTAGACGTTTCCCGC |
| Internal bar code: | TGAAGCCGCCTTGTCGTGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 809 |
| LEAP-Seq percent confirming: | 98.8849 |
| LEAP-Seq n confirming: | 3015 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGTACCGTATGTGCGTG |
| Suggested primer 2: | TGTTTGAGGATGCTCAGGTG |