| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145195 |
| Chromosome: | chromosome 3 |
| Location: | 5562306 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186500 | HAT2 | Histone acetyltransferase 2; (1 of 1) K11303 - histone acetyltransferase 1 (HAT1, KAT1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCGATTGAGCCTTGGTACCGCGTTACC |
| Internal bar code: | GAATGGCCGGGTCCGAGTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 309 |
| LEAP-Seq percent confirming: | 99.7006 |
| LEAP-Seq n confirming: | 999 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTACGCCTTCACTTCTT |
| Suggested primer 2: | CCCAGCCCCTACCAGTAAAT |