| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145206 |
| Chromosome: | chromosome 14 |
| Location: | 2483555 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625050 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACACATATTATATCGGATACCGGTTG |
| Internal bar code: | CAGAGATGTCGCTCGCGGTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 99.38 |
| LEAP-Seq n confirming: | 1603 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTCCAAGCTGACAAGCCT |
| Suggested primer 2: | CCAGGTTGAGGTTGACCAGT |