| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145225 |
| Chromosome: | chromosome 13 |
| Location: | 614036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565750 | EFG4 | (1 of 1) K14416 - elongation factor 1 alpha-like protein (HBS1); Translation elongation factor Tu family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTCAGGGACCCAAGAGGCAATCAACCA |
| Internal bar code: | TATACCTCTCCTAATAGAGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 99.6519 |
| LEAP-Seq n confirming: | 16033 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGACCGACCGTCTACCTC |
| Suggested primer 2: | ACAATGTGGTGTGGTGTGCT |