| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145229 |
| Chromosome: | chromosome 12 |
| Location: | 4007951 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516950 | BLD12,SAS6 | (1 of 1) K16487 - spindle assembly abnormal protein 6 (SAS-6, SASS6); Bald 12 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGTCGTCGAGAAGAAGCGGCATTTCGAG |
| Internal bar code: | TGCGCAGGATCGTCTCCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 543 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAAACACGGATGCAATGT |
| Suggested primer 2: | CATCAGACCCACCGATTTCT |