Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.145229 |
Chromosome: | chromosome_12 |
Location: | 4008079 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g516950 | SAS6 | Basal body cartwheel protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGGCGTGGCAAAGCAAAACCACAACCTTCG |
Internal bar code: | AAGGACTCGGGCTCGCAACTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 594 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 134 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAACCCAGAGTATTGGCAT |
Suggested primer 2: | TCCACAATCTTGAACACGGA |