| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145271 |
| Chromosome: | chromosome 7 |
| Location: | 2714581 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331114 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCACACTTTACAGTCCTCACCAACGCCA |
| Internal bar code: | ACGGGTATTAGCCACCGCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 867 |
| LEAP-Seq percent confirming: | 99.8438 |
| LEAP-Seq n confirming: | 5115 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACCCAGTCCTCAGACC |
| Suggested primer 2: | GTGACGGACCAGGCCTATTA |