| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145277 |
| Chromosome: | chromosome 13 |
| Location: | 4987995 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606600 | CGK1 | cGMP-dependent protein kinase; (1 of 6) K07376 - protein kinase, cGMP-dependent (PRKG) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTTTCAGCCAGTGCTGCGCAGCACCGC |
| Internal bar code: | GCGGCAGTTTAGGGATGCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCGCTGTTGTGACCTGA |
| Suggested primer 2: | ACCAAAGCGGGTTCTTACCT |