| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145279 |
| Chromosome: | chromosome 1 |
| Location: | 4743589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032800 | MRS2,MRS6 | Mg2+ transporter protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGTCTGGGACGCCGTGTCGGCCATGC |
| Internal bar code: | TAGGAAGAAGAAGCGTCTTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 78.0105 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGTACGCACCCATAAG |
| Suggested primer 2: | ACCCTGACAGGTAGTGGTGC |