| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145305 |
| Chromosome: | chromosome 12 |
| Location: | 2565905 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505400 | (1 of 1) 3.1.1.47 - 1-alkyl-2-acetylglycerophosphocholine esterase / Platelet-activating factor acetylhydrolase; Related to platelet-activating factor acetylhydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTGATGACCGGGCTCTGCTTCGGACTG |
| Internal bar code: | CAATAGCTTGGTAGCATGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.7485 |
| LEAP-Seq n confirming: | 12297 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGTTCACGCAGGTTCAA |
| Suggested primer 2: | CAGGCGACATTAGCTGCATA |