| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145312 |
| Chromosome: | chromosome 16 |
| Location: | 951849 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648700 | (1 of 4) 3.6.3.29 - Molybdate-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTTGCGCTCCCATCCATCCACTCCATC |
| Internal bar code: | GTCTGCTTTTCAGGACACTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 119 |
| LEAP-Seq percent confirming: | 98.9677 |
| LEAP-Seq n confirming: | 767 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCCGTAGAAGGTGAAGG |
| Suggested primer 2: | AACCGCAACTGAAATGGAAC |