Insertion junction: LMJ.RY0402.145345_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.145345
Chromosome:chromosome 6
Location:4345003
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278263 (1 of 4) 2.3.1.84 - Alcohol O-acetyltransferase / AATASE CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTCGCGGCCAAGACGCGCGTTGCCCCCG
Internal bar code:GACAGAGGACATAGAGGAGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:354
LEAP-Seq percent confirming:99.7188
LEAP-Seq n confirming:6384
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCTGTAAGTGCTCTCCC
Suggested primer 2:CGGCAATAGACAGCAACAGA