Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.145351 |
Chromosome: | chromosome 12 |
Location: | 5882160 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g533900 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTTGCCGGTTTCAGCTCGTTGATCTAG |
Internal bar code: | CGGACTCCCGAACGGCGTGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 835 |
LEAP-Seq percent confirming: | 99.8844 |
LEAP-Seq n confirming: | 6911 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAAACCTGGTGCTTTCATT |
Suggested primer 2: | CGGCGTAAAAAGGTGTTCAT |