| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145370 |
| Chromosome: | chromosome_7 |
| Location: | 2612689 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g330200 | RSP9 | Radial spoke protein 9 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGGGTGCATTGGGCAGCCGTCCTTTTCAC |
| Internal bar code: | CAATTAACAACTTTCGATCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1101 |
| LEAP-Seq percent confirming: | 99.646 |
| LEAP-Seq n confirming: | 3941 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGGTGCCTAAAACAGAT |
| Suggested primer 2: | TGCCTGCGGAACATATTACA |