| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145429 |
| Chromosome: | chromosome 8 |
| Location: | 73232 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358524 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGGGTAGCAGCGCGAGTGGAGACACGC |
| Internal bar code: | ATCGCGGAGGTGACGCTCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 79.5476 |
| LEAP-Seq n confirming: | 5768 |
| LEAP-Seq n nonconfirming: | 1483 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACATGACGCTAGACGAGG |
| Suggested primer 2: | AAGACACTGGCACACGACAC |