Insertion junction: LMJ.RY0402.145433_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.145433
Chromosome:chromosome 12
Location:5467609
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g530750 (1 of 239) IPR016024 - Armadillo-type fold 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCTTTGGGTGACGTGGTGTGCTCGGGGG
Internal bar code:CCCCGCGCGGGTCCCAGAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1002
LEAP-Seq percent confirming:99.6647
LEAP-Seq n confirming:27646
LEAP-Seq n nonconfirming:93
LEAP-Seq n unique pos:161

Suggested primers for confirmation by PCR

Suggested primer 1:AAATTGCATGCAGCTCACAC
Suggested primer 2:AGGGCAACAAACACTCAACC