Insertion junction: LMJ.RY0402.145441_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.145441
Chromosome:chromosome 2
Location:6496446
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g116400 FAP120,DII7 Flagellar Associated Protein 120; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCAACGGGCTGCGCTTCCGCTTGCTCCT
Internal bar code:TAAGTCAGGCGACGACGAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:665
LEAP-Seq percent confirming:98.1627
LEAP-Seq n confirming:374
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TACACAGCCGTTTGGTGGTA
Suggested primer 2:ATTGACGTCTTCAAAACGGG