| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.145465 |
| Chromosome: | chromosome 12 |
| Location: | 3913420 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515950 | (1 of 35) IPR001763 - Rhodanese-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCGTTGGCGTGCATGCGCACAGCCACC |
| Internal bar code: | AATGCTCTACGTCAGTGGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 911 |
| LEAP-Seq percent confirming: | 99.7558 |
| LEAP-Seq n confirming: | 4493 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTTGTAGCATAAGCGGG |
| Suggested primer 2: | TCATGTAGCGCGTCTTCAAC |