| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145482 |
| Chromosome: | chromosome_5 |
| Location: | 2240056 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g234661 | BCS1 | Ubiquinol:cytochrome c oxidoreductase biogenesis factor | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATAAGTCGAGCCAGTGCTGATCTTAGACA |
| Internal bar code: | TCCCCTATTGATTAATGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 99.5458 |
| LEAP-Seq n confirming: | 1315 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGCCTTTCTACCTTCATC |
| Suggested primer 2: | GCAGCCTCAGATACGACACA |