| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145482 |
| Chromosome: | chromosome_10 |
| Location: | 1510985 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g428850 | DLT1,DLC9,LC9 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGGGAATTAAGCAGGCTCCGTGACTGCTG |
| Internal bar code: | AGGATACGGGTTAGCGCTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 97.7579 |
| LEAP-Seq n confirming: | 436 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGGTAGCTTTGTTTTGC |
| Suggested primer 2: | CCCAAGTTCTACCCGCTACA |