| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145489 |
| Chromosome: | chromosome 13 |
| Location: | 4864160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605650 | ALDH10,ALDH10A1,ALD7 | (1 of 1) 1.2.1.8 - Betaine-aldehyde dehydrogenase / Betaine aldehyde oxidase; Aldehyde dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGAGCTGGCCTCGCTCACCTGCCTGGAG |
| Internal bar code: | CATCGACACTACATCGCCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 146 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 126 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTACATCCCAAACCCCCTT |
| Suggested primer 2: | GTTGCCCTGCTACTGCTAGG |