| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145498 |
| Chromosome: | chromosome 2 |
| Location: | 3641230 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095146 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACCATCATTGCCCCCTCACCGTGCTA |
| Internal bar code: | CACGCTCAATGCGGTGACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 99.5568 |
| LEAP-Seq n confirming: | 6514 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTTCATCACCCACACC |
| Suggested primer 2: | AGGTGTGTGTGCGTGCTTAG |