| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145507 |
| Chromosome: | chromosome 12 |
| Location: | 6457002 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538150 | (1 of 1) K05019 - chloride channel, nucleotide-sensitive, 1A (CLNS1A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATGTGCGGAGCGGCGGCACGGCACAG |
| Internal bar code: | GGGACTCTCGCCTTTCGCACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 98.3455 |
| LEAP-Seq n confirming: | 2972 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACGGGGTGTACGGTATT |
| Suggested primer 2: | TTTGAATTCTCCCGTCTTGG |