| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145516 |
| Chromosome: | chromosome 16 |
| Location: | 1233903 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651000 | RFA3,RPA70B | (1 of 1) PTHR23273:SF4 - REPLICATION PROTEIN A 70 KDA DNA-BINDING SUBUNIT; Replication protein A, 70 kDa DNA-binding subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCCCCTGCACACCCACCCACCTGGAAG |
| Internal bar code: | CGCGGTGAGCTAAGTGAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 97.5761 |
| LEAP-Seq n confirming: | 1892 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACCTCAAATTACAGCCC |
| Suggested primer 2: | CATCACTGTTCGCTCCTTGA |