| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145526 |
| Chromosome: | chromosome 17 |
| Location: | 6872228 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g745547 | (1 of 1) PF05182 - Fip1 motif (Fip1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAAGAGGAGGAGCGGGAGGAGGGGCC |
| Internal bar code: | GCACACCACACAGACATCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 99.8016 |
| LEAP-Seq n confirming: | 1006 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCACCCTGGATGAATTGG |
| Suggested primer 2: | AAGGGGAACACTAACCCCAC |