| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145531 |
| Chromosome: | chromosome 2 |
| Location: | 6489539 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116300 | (1 of 1) K15171 - transcription elongation factor SPT4 (SUPT4H1, SPT4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACGCACAAAGGTTGCAATGCCGCGCAA |
| Internal bar code: | TTTCTTTTCGGAACCTAGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 98.4906 |
| LEAP-Seq n confirming: | 1305 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCTGTGACAGGATGAGC |
| Suggested primer 2: | GCACCTACTTGGACTCGCTC |