| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.145539 |
| Chromosome: | chromosome 16 |
| Location: | 7525469 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687966 | FAP5,Nphp3 | Flagellar Associated Protein 5; (1 of 2) PTHR19959//PTHR19959:SF147 - KINESIN LIGHT CHAIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTGCTGTGAGTAACGGTCCCCTGCGTGC |
| Internal bar code: | CGGCCGCTTAGAGATCAGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 98.8811 |
| LEAP-Seq n confirming: | 3535 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGACAGAGGTTATGGGAT |
| Suggested primer 2: | GAGCCGAGAGTTGCATTAGG |