Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.145558 |
Chromosome: | chromosome 10 |
Location: | 5184981 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g456800 | (1 of 2) PF09733 - VEFS-Box of polycomb protein (VEFS-Box) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCAGGCAGCGGCGGCGACGGCTCCGGG |
Internal bar code: | TGTCCGTGCCGAGGCAATGCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 302 |
LEAP-Seq percent confirming: | 99.7691 |
LEAP-Seq n confirming: | 432 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTTCACCCACCCTTCACT |
Suggested primer 2: | GCAAGTTGTAATGCTTCGCA |