Insertion junction: LMJ.RY0402.145563_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.145563
Chromosome:chromosome 3
Location:220133
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g144344 (1 of 1) IPR003613//IPR011009//IPR013083//IPR013320 - U box domain // Protein kinase-like domain // Zinc finger, RING/FYVE/PHD-type // Concanavalin A-like lectin/glucanase domain intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGCCCATCCACGGGTTGGCGCTCCTCC
Internal bar code:CAAACCTCGATTAGGGCAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:930
LEAP-Seq percent confirming:99.6294
LEAP-Seq n confirming:1613
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAGTAAGGCGGTCTAGCG
Suggested primer 2:TATGGTGATCCCTGCTCCTC