| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.145582 |
| Chromosome: | chromosome 5 |
| Location: | 2654731 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236500 | (1 of 1) 3.5.1.23 - Ceramidase / Acylsphingosine deacylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCAACTGCCCAGCGCTCACCTGAAAGT |
| Internal bar code: | TTAGGTTGTCTATCTCTGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 99.7523 |
| LEAP-Seq n confirming: | 4027 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTATTGCCGTTCAGGGT |
| Suggested primer 2: | CTGCTCTCACCTGTCCCAAT |