| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.145656 |
| Chromosome: | chromosome 8 |
| Location: | 2294642 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370200 | (1 of 5) IPR001214//IPR003616 - SET domain // Post-SET domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGTCCGGTGATGTCCTCAGCAGCGTCA |
| Internal bar code: | CCACCACCGGTCGGTTGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 97.2644 |
| LEAP-Seq n confirming: | 1600 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAGTCGTGGTTACTTCC |
| Suggested primer 2: | GTGCGTGGTTGATGATAACG |